Liquid biopsy uses bodily fluids to assess health status, most often employed for the detection of cancer presence in plasma. Tissue biopsy is the current gold standard, but it is not always available, or it can be very difficult to obtain (e.g. in lung cancer).

A liquid biopsy may be used to help find cancer at an early stage, as well as to help plan treatment, to find out how well treatment is working or if cancer has come back. Taking multiple samples of blood over time may also help doctors understand what kind of molecular changes are taking place in a tumour and how this might influence therapy.

What is liquid biopsy?

Liquid biopsy uses bodily fluids to assess health status, most often employed for the detection of cancer presence in plasma. As a target, circulating tumour DNA (ctDNA), circulating tumour cells and many other substances can be used (Fig.1), of which ctDNA is most broadly used one.

[.NL-en Netherlands (english)] Liquid biopsy

Tissue biopsy is the current gold standard, but it is not always available, or it can be very difficult to obtain (e.g. in lung cancer). It is also not practical to routinely use tissue samples for follow-up of patients. In contrast, liquid biopsy is a convenient and non-invasive method to obtain such information for advancing clinical studies. Because of this, liquid biopsy has now been included in some cancer guidelines already, including those for lung, colorectal and breast cancers.

Our OncoBEAM™ technology has been the most sensitive technology available by almost factor 20x versus other routine technologies such as PCR (polymerase chain reaction) and conventional NGS (next-generation sequencing). SafeSEQ, the technology building the basis of the new Plasma-SeqSensei™ product line, offers similar sensitivity combined with higher workflow convenience. The sensitivity is achieved by barcoding each sample, thereby reducing error rates by >100x.

Why use liquid biopsy?

High concordance with tissue samples in most cancers
Minimally invasive, tissue samples may be difficult to obtain
More cost-effective than tissue biopsy
More convenient for clinicians and patients
Holistic tumour information (tissue samples may not be representative due to a sampling error)
Faster turnaround time (TAT) compared to tissue sample handling
Repeat sampling possible in contrast to tissue samples
Real-time information

Why use Plasma-SeqSensei™?

Highly sensitive – able to detect 7 MM (mutant molecules) with 95 % confidence across all mutations (absolute quantification) and corresponding to a sensitivity of down to 0.07 % MAF (mutant allele frequency, approx. 40 % of samples have MAF below detection limit of most technologies)
High flexibility of between 2 - 16 samples/run
High laboratory efficiency as several different types of samples may be processed in one run
Standardised workflow, high reproducibility
Fast TAT (2 days)
Convenient software

Our liquid biopsy solutions

Plasma-SeqSensei™ Breast Cancer IVD Kit

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Plasma-SeqSensei™ Colorectal Cancer RUO Kit

Plasma-SeqSensei™ CRC RUO Kit

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Plasma-SeqSensei™ Non-Small Cell Lung Cancer RUO Kit

Plasma-SeqSensei™ NSCLC RUO Kit

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References

[1] National Cancer Institute (2022): liquid biopsy

[2] Schmiegel et al. (2017): Mol Oncol. 11(2) : 208 – 219

[3] Saunders et al. (2016): Annals of Onc 27 (6) : 149 – 206

[4] Vidal et al. (2017): J Clin Oncol 35 (suppl 4S; Abstract 607)

[5] Oxnard et al. (2016): J Clin Oncol 34(28):3375-3382

[6] Baselga et al. (2015): Oral pres. SABCS, Abstract S 6 – 01

Plasma-SeqSensei RUO Kit^TM is intended for Research Use Only. Diagnostic use is not supported.