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CyFlow™ CD10 Alexa Fluor™ 700

Alternative Name: CALLA, NEP, gp100
Antigen: CD10
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-78
Emission Maximum: 723 nm
Excitation Maximum: 702 nm
Field of Interest: Immunophenotyping
Fluorochrome: Alexa Fluor™ 700
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Bestelnummer: AN915206
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CyFlow™ CD10 Alexa Fluor™ 700
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HLDA WorkshopHLDA IV—WS Code B 506 HLDA V—WS Code B CD10.4
Quantity100 tests
Volume0.4 mL
ImmunogenNALM-6 human pre-B cell line
Background InformationCD10 (neutral endopeptidase - NEP, common acute lymphocytic leukemia antigen - CALLA, membrane metallo-endopeptidase - MME, enkefalinase) is a 100 kDa cell surface zinc metalloprotease cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptides. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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